Título: Behavior Exploration for Model Selection of Image Based Result Quantification of Newly Developed HIV, HCV and FOB Lateral Flow Device Tests
Autores: Fontes, Cassio Mendes; Braga, Antônio de Pádua
Resumo: Lateral Flow immunoassays, have been developed in the early eighties and due to its characteristics such as low cost, unnecessary use of costly readers for obtaining a qualitative result, easy to use and to interpret formats, they became very popular worldwide. Due to their unique characteristics, they allowed health organization to bring point-of-care testing to populations that have rarely seen medical care. Although several advantages may be pointed out, there are two main set backs on the technology; the lack of quantification possibilities for the test devices and possible human error, which may occur in weak signal results. Since most of the tests use colloidal gold conjugates, reading systems for such tests depend on image based recognition for final result quantification or assured qualitative results. In this work we not only developed three lateral flow tests, for the detection of HIV, HCV and occult blood in feces (FOB), but we analyzed images from final tests, in order to access the possibility of quantifying the test’s final result and encountered a linear relation between the final result and the amount of analyte present in the sample. Although simple, such solution can broaden the applications of lateral flow technology.
Código DOI: 10.21528/CBIC2013-289
Artigo em pdf: bricsccicbic2013_submission_289.pdf
Arquivo BibTex: bricsccicbic2013_submission_289.bib